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LRE qPCR Overview Part 2-Optical Calibration

Part 2 of the LRE qPCR overview series, in which optical calibration is introduced, along with how it can be used to convert target quantity expressed in fluorescence units into the number of target molecules. Note that a fully featured desktop application that automates LRE quantification is now available at https://sites.google.com/site/lreqpcr/program-files

Science > Molecular Biosciences > Molecular Diagnostics and Therapeutics

Science > Molecular Biosciences > Molecular Biology

Keywords: qPCR, real-time qPCR, absolute quantification, LRE qPCR, Gene Expression Profiling, gene expression analysis, DNA quantification

Posted by: Bob Rutledge

Posted on: 15 Jan 2014

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