Mapping low-frequency field potentials in cortico-hippocampal brain slices with high-resolution CMOS-MEAs
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1
Fondazione Istituto Italiano di Technologia, Nbt, Italy
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2
3Brain GmbH, Switzerland
Technology advances in MEA technology led to large-scale electrode array CMOS devices capable of simultaneously recording from thousands of closely spaced microelectrodes for high spatial and temporal resolution monitoring of bioelectrical signals in cell cultures and brain circuits ex-vivo. In brain slices, this technology can provide sub-millisecond and micrometric resolutions over a wide field of view (2.7 x 2.7 mm2, 4096 electrodes) to record both low frequency field potential and spiking activities. In this work, I will present results obtained on cortico-hippocampal brain slice preparations toward two directions, both aiming at exploiting the provided spatio-temporal resolution. First, I have investigated its use for compound testing by developing computational and experimental methods to quantify compound’s effects on LTP. Results show that automatic clustering methods let to spatially map differently potentiated anatomical regions. By considering this spatial information on LTP we could achieve significant quantifications for compounds that were showing unclear effects when tested with conventional setups based on two micro-positioned electrodes. Second, I started investigating the use of this technology to record and to characterize electrically induced and spontaneous sharp waves ripples. Both studies reveal the potential of exploiting high-resolution electrophysiological CMOS-MEAs for studies and applications on low-frequency field potentials.
Keywords:
CMOS-MEAs,
Electrophysiology,
LTP,
SPW-R,
Electrical Stimulation
Conference:
MEA Meeting 2018 | 11th International Meeting on Substrate Integrated Microelectrode Arrays, Reutlingen, Germany, 4 Jul - 6 Jul, 2018.
Presentation Type:
Poster Presentation
Topic:
Microelectrode Array Technology
Citation:
Zordan
S,
Lonardoni
D,
Maccione
A and
Berdondini
L
(2019). Mapping low-frequency field potentials in cortico-hippocampal brain slices with high-resolution CMOS-MEAs.
Conference Abstract:
MEA Meeting 2018 | 11th International Meeting on Substrate Integrated Microelectrode Arrays.
doi: 10.3389/conf.fncel.2018.38.00117
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Received:
17 Mar 2018;
Published Online:
17 Jan 2019.
*
Correspondence:
Mr. Stefano Zordan, Fondazione Istituto Italiano di Technologia, Nbt, Genoa, Italy, stefano.zordan@iit.it