Carbon Nanotube Microelectrode Array for Real-time Measurement of Dopamine Release in Human iPS Cell-derived neuron
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1
Tohoku Institute of Technology, Department of Electronics, Japan
Motivation
Multi-electrode array (MEA) assays using human induced pluripotent stem cell (hiPSC)-derived neurons are expected to predict the toxicity and the pharmacological effects[1]. If we can measure the release of neurotransmitter using this MEA, it is possible to evaluate the drug related to release of neurotransmitters, and it is expected to improve the accuracy of medicinal effects. In this study, we aimed to develop the carbon nanotube (CNT) MEA chip, which enables real-time measurement of neurotransmitters by electrochemical reaction.
Material and Methods
The CNT-MEA chip was fabricated by electro-plating method. Detection sensitivity to dopamine (DA) in the fabricated CNT-MEA chip was examined by electrochemical measurement method. The change of DA release to methamphetamine (MTH) were measured using mounted striatal slice of 6 weeks old mice and cultured human iPSC-derived dopamine neurons on CNT-MEA.
Results
As a result of the electrochemical measurement, an oxidation peak current was observed at 0.25 V, and the detection limit and linearity of DA was less than 1 nM. We have succeeded in real time detection of DA release using striatal slices and human iPS cell derived DA neurons, and detected the changes in the amout of DA release depending on MTH dose. Futhremore, in the human iPSC-derived DA neuron, a change of spike pattern at MTH administration was detected by conventional filed potential measurement.
Discussion and Conclusion
We developed a novel CNT-MEA chip having high sensitivity to DA and enebling the detection of DA release from brain slices and cultured hiPSC-derived neuronal networks. We also found that CNT-MEA is possible to two measurements, electrochemical measurement of DA release and conventional field potential measurement. CNT-MEA is expected as a new MEA measurement method that improves the accuracy of toxicity prediction and the pharmacological effects.
References
[1] Odawara A, Katoh H, Matsuda N, Suzuki I. Physiological maturation and drug responses of human induced pluripotent stem cell-derived cortical neuronal networks in long-term culture. Sci Rep. 6, 26181 (2016) doi:10.1038/srep26181
Keywords:
Carbon nanotube (CNT),
hiPSC,
Dopamine,
Electrochemical Techniques,
Real-time measurement
Conference:
MEA Meeting 2018 | 11th International Meeting on Substrate Integrated Microelectrode Arrays, Reutlingen, Germany, 4 Jul - 6 Jul, 2018.
Presentation Type:
Poster Presentation
Topic:
Microelectrode Array Technology
Citation:
Matsuda
N,
Odawara
A and
Suzuki
I
(2019). Carbon Nanotube Microelectrode Array for Real-time Measurement of Dopamine Release in Human iPS Cell-derived neuron.
Conference Abstract:
MEA Meeting 2018 | 11th International Meeting on Substrate Integrated Microelectrode Arrays.
doi: 10.3389/conf.fncel.2018.38.00104
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Received:
18 Mar 2018;
Published Online:
17 Jan 2019.
*
Correspondence:
Mr. Naoki Matsuda, Tohoku Institute of Technology, Department of Electronics, Sendai, miyagi, Japan, m161802@st.tohtech.ac.jp