Differential regulation of hypothalamic neuropeptide Y and aguti-related protein expression during psychological stress and insulin-induced hypoglycemia
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1
Institute of Experimental Medicine, Hungary
Many signals reflecting energy balance and stress are integrated at the hypothalamic orexigenic NPY and AgRP neurons. To determine transcriptional changes of the npy and agrp gene in response to stress challenges, we followed the expression of heteronuclear (hn)- and messenger (m)RNA levels by in situ hybridization histochemistry and by Real-Time qPCR assays in mice following insulin-induced hypoglycaemia and restraint. Hypoglycaemia in fasted mice resulted in a rapid increase of NPY hnRNA that peaked at 1 hour, declined thereafter by 2-4 hours after insulin injection and run parallel to that of NPY mRNA. Throughout the time course examined, NPY expressing cells in the medial-basal hypothalamus remained overwhelmingly localized to the arcuate nucleus. Following restraint stress, NPY mRNA signal was increased however hnRNA levels decreased, suggesting increased stability of mature NPY mRNA. However in the brainstem the NPY hnRNA peaked at 120 min after insulin administration. Restraint increased the NPY hnRNA level at 30 min after the stress and declined thereafter by 2-4 hours. The AgRP hnRNA levels remain unchanged during hypoglicemia contrary the mRNA peaked at 60 min in the hypothalamus after insulin injection. Restraint increased AgRP mRNA level at 60 min.and hnRNA level at 120 min after the end of the stress. These results highlight rapid changes and differential regulation of NPY and AgRP expression in response to metabolic and stress challenges.
Keywords:
Neuroendocrinology,
Neuroscience
Conference:
13th Conference of the Hungarian Neuroscience Society (MITT), Budapest, Hungary, 20 Jan - 22 Jan, 2011.
Presentation Type:
Abstract
Topic:
Neuroendocrinology
Citation:
Ferenczi
S,
Zelei
E,
Pintér
B and
Kovács
KJ
(2011). Differential regulation of hypothalamic neuropeptide Y and aguti-related protein expression during psychological stress and insulin-induced hypoglycemia.
Front. Neurosci.
Conference Abstract:
13th Conference of the Hungarian Neuroscience Society (MITT).
doi: 10.3389/conf.fnins.2011.84.00119
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Received:
03 Mar 2011;
Published Online:
23 Mar 2011.
*
Correspondence:
Dr. K. J Kovács, Institute of Experimental Medicine, Budapest, Hungary, kovacs@koki.hu