Changes in miRNA expression profile between stress-vulnerable and stress-resilient rats in Chronic Mild Stress (CMS) - an animal model of depression.
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1
Institute of Pharmacology Polish Academy of Sciences, Department of Pharmacology Laboratory of Biochemical Pharmacology, Poland
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2
Institute of Pharmacology Polish Academy of Sciences , Department of Pharmacology Laboratory of Behavioural Pharmacology, Poland
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3
Institute of Pharmacology Polish Academy of Sciences, Department of Pharmacology Laboratory of Behavioural Pharmacology, Poland
Prolonged, chronic stressful events are considered to be one of the major causes of depression, however many people and animals exposed to stress seem to be resilient. Only a few studies have focused on stress resilience phenomenon. Depression may result from impairment in neuronal plasticity which may be associated with altered miRNA expression level - a class of newly discovered posttranscriptional regulators of gene expression. We used Chronic Mild Stress (CMS) paradigm which is well characterised animal model of depression. We observed that in this model about 30% of rats exposed to chronic, stressful conditions demonstrate resilience to stress what is manifested as not altered consumption of a palatable 1% sucrose solution by this group of animals. We investigated a global miRNA expression profile in serum of rats subjected to chronic mild stress to identify the potential, non-invasive molecular markers differentiating animals that demonstrated resilience to stress from stress-reactive ones. After two weeks of CMS procedure we identified three groups of rats based on their sucrose consumption: control (12,1±0,6g), anhedonic (5,3±0,4g, p<0,0001) and stress resilient (11,6±0,4g,). Animals were sacrificed and the serum was collected, from which miRNA was purified. The RNA concentration and quality was measured with NanoDrop spectrophotometer. RT-PCR based TaqMan Array Rodent MicroRNA Cards were used to screening the changes in miRNA expression induced by stress conditions and response to stress. Using Expression Suite Software analysis we found that stress significantly altered the expression of one miRNA in anhedonic rats as compared to control group, while in stress-resilient animals the expression of 16 miRNAs was changed. This data indicate that resilient animals can actively cope with stressful conditions leading to "žstress-resistant" phenotype. miRNAs may play important role in stress resilience by pleiotropic modulation of expression of many genes.
The study was supported by National Science Center Grant UMO-2012/07/B/NZ7/01164, Poland.
Keywords:
Depression,
miRNA,
Animal Models,
Chronic mild stress,
stress-resilience
Conference:
XII International Conference on Cognitive Neuroscience (ICON-XII), Brisbane, Queensland, Australia, 27 Jul - 31 Jul, 2014.
Presentation Type:
Poster
Topic:
Emotional and Social Processes
Citation:
Zurawek
D,
Faron-Gorecka
A,
Kusmider
M,
Kolasa
M,
Pabian
P,
Solich
J,
Szafran
K,
Gruca
P,
Papp
M and
Dziedzicka-Wasylewska
M
(2015). Changes in miRNA expression profile between stress-vulnerable and stress-resilient rats in Chronic Mild Stress (CMS) - an animal model of depression..
Conference Abstract:
XII International Conference on Cognitive Neuroscience (ICON-XII).
doi: 10.3389/conf.fnhum.2015.217.00376
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Received:
19 Feb 2015;
Published Online:
24 Apr 2015.
*
Correspondence:
Mr. Dariusz Zurawek, Institute of Pharmacology Polish Academy of Sciences, Department of Pharmacology Laboratory of Biochemical Pharmacology, Krakow, Poland, zurawek@if-pan.krakow.pl