Protective effects of alpha lipoic-acid and n-acetyl cysteine against acrylamide-induced peripheric neuropathy
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1
Marmara University, Vocational School of Health Related Services, Department of Medical Laboratory Techniques, Türkiye
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2
Department of Pharmacology, Faculty of Pharmacy, Marmara University, Türkiye
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3
Department of Histology and Embryology, School of Medicine, Marmara University, Türkiye
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4
Department of Biochemistry, School of Medicine, Marmara University, Türkiye
Acrylamide (ACR) is a vinyl monomer with a significantly high chemical reactivity. It is easily absorbed by all routes of administration and peripheral nervous system is a selective target for its toxicity. The early clinical symptoms to ACR results in degeneration of nerves causing distal axonopathy. ACR has been reported to cause disturbances in oxidative stress. The aim of this study is to determine the effects of alpha lipoic-acid (LA) and n-acetyl cysteine (NAC) against acrylamide-induced peripheric neuropathy in rats.
Male Sprague-Dawley rats were included in the study. ACR was given i.p. at a dose of 45mg/kg/day. LA group received additionally 35mg/kg/day LA, and NAC group received 150mg/kg/day NAC for 10 days. Controls were injected saline at the same dose. After the time rats were sacrified and sciatic nerves were removed. For free radical determination luminol (selective for hydroxyl radical, hydrogen peroxide and hypochlorous acid) and lucigenin (selective for superoxide radical) enhanced chemiluminescence (CL) method was used. Additionally histopathological examination was also made.
Our luminol enhanced CL results was in ACR group higher than control group (21,6±7,1rlu/mg tissue vs. 11,2±1,9rlu/mg tissue;p<0,001). LA and NAC reduces luminol enhanced CL (8,6±1,7rlu/mg tissue vs. 9,5±2,2rlu/mg tissue) measurements significantly (p<0,001). Lucigenin enhanced CL measurements did not changed between ACR, control and NAC received group (p>0.05). LA has a low reducing effect but was also not significantly. Histopathological examination has shown normal myelination in peripheric neurons. In ACR group myelin organization was damaged and the count of disturbed myelinated nerve fibers was increased with respect to the control group. NAC and LA groups has shown a moderate reducing effect against myelin damage.
ACR toxicity increased oxidative stress in peripheric neurons, which results with myelin damage. NAC and LA can reduce this damage. In conclusion, LA and NAC has protective effects against ACR induced peripheric neuropathy.
Acknowledgements
This project was supported by the Marmara University Research Fund (SAG-BGS-081004-0108).
Keywords:
Acrylamide,
alpha-lipoic acid,
n-acetyl cysteine,
Neuropathy,
Free Radicals
Conference:
4th Conference of the Mediterrarnean Neuroscience Society, Istanbul, Türkiye, 30 Sep - 3 Oct, 2012.
Presentation Type:
Poster Presentation
Topic:
Abstracts
Citation:
Yuksel
M,
Şehirli
A,
Tok
EO,
Ercan
F and
Haklar
G
(2013). Protective effects of alpha lipoic-acid and n-acetyl cysteine against acrylamide-induced peripheric neuropathy.
Conference Abstract:
4th Conference of the Mediterrarnean Neuroscience Society.
doi: 10.3389/conf.fnhum.2013.210.00073
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Received:
27 Mar 2013;
Published Online:
11 Apr 2013.
*
Correspondence:
Dr. Meral Yuksel, Marmara University, Vocational School of Health Related Services, Department of Medical Laboratory Techniques, Istanbul, Türkiye, meralyuksel@gmail.com