Caffeine and modafinil modulate inflammatory proteins correlating cognitive impairment during 48h of total sleep deprivation in rat hippocampus
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1
Defence Institute of Physiology and Allied Sciences, DRDO, Department of Neurophysiology, India
Sleep loss has pro-inflammatory effect and decrease anti-inflammatory proteins level in the hippocampus. It has been reported that psychostimulant drugs modulate the inflammatory molecules. We examined the modulatory role of modafinil (100 mg/kg/day, orally) and caffeine (60 mg/kg/day, orally) on behavioral and molecular level during 48h of (SD). A novel automated cage shaking stimulus was used to induce SD. Rats were subjected to the following behavioral tests: novel object recognition test (NORT), Morris water maze (MWM), elevated plus maze (EPM) and forced swim test (FST). Hippocampal tissues were collected for biochemical and immunohistochemical studies. SD induced a deficit in spatial memory, recognition memory and showed anxious state, but did not show depressive behaviour. Caffeine/modafinil administration significantly improved these behavioural consequences. SD disturbs the inflammatory balance (increased pro-inflammatory/decreased anti-inflammatory cytokines) while treatment with caffeine/modafinil shift this balance towards anti-inflammatory side. Stereological analysis showed that SD increased the number of activated microglial cells and caffeine/modafinil treatment significantly improved the resting state microglia cells number during SD. Caffeine and modafinil treatment prevented the SD induced up-regulation of expression of Iba-1 and glial fibrillary acidic protein (GFAP) and improved their immunoreactivity in hippocampus after SD. The results suggested that caffeine/modafinil administration preserved basal anti-inflammatory strength, which facilitated cognitive performance.
Keywords:
Cognition,
Hippocampus,
in vivo,
Neuroinflammation,
behavioural assessment
Conference:
14th Meeting of the Asian-Pacific Society for Neurochemistry, Kuala Lumpur, Malaysia, 27 Aug - 30 Aug, 2016.
Presentation Type:
Poster Presentation Session
Topic:
14th Meeting of the Asian-Pacific Society for Neurochemistry
Citation:
Wadhwa
M,
Jain
V,
Roy
K,
Chauhan
G and
Panjwani
U
(2016). Caffeine and modafinil modulate inflammatory proteins correlating cognitive impairment during 48h of total sleep deprivation in rat hippocampus.
Conference Abstract:
14th Meeting of the Asian-Pacific Society for Neurochemistry.
doi: 10.3389/conf.fncel.2016.36.00192
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Received:
04 Aug 2016;
Published Online:
11 Aug 2016.
*
Correspondence:
Dr. Usha Panjwani, Defence Institute of Physiology and Allied Sciences, DRDO, Department of Neurophysiology, Delhi, Delhi, India, neurophysiolab.dipas@gmail.com