Protective effect of melatonin on methamphetamine-induced synaptic degeneration in postnatal hippocampus
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1
Srinakharinwirot University, Department of Anatomy, Faculty of Medicine, Thailand
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2
Institute of Molecular Biosciences, Mahidol University, Research Center for Neuroscience, Thailand
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3
Mahidol University, Center for Neuroscience, Faculty of Science, Thailand
Methamphetamine (METH), an addictive recreational drug, strongly stimulates central nervous system. Chronic administration of METH can induce damage in several brain areas. Previous studies showed that METH induced loss of dendritic complexity and neuronal cell body and increase of reactive oxygen species formation in the hippocampus. Hippocampus is an area in the temporal lobe that plays a major role in spatial learning and memory. Melatonin, a hormone that is released from the pineal gland, plays a role in regulation of circadian rhythms with a powerful anti-oxidation property. Many studies have revealed that melatonin can protect neurons from METH-induced degeneration in various areas. Therefore, the aim of this study is to examine the effect of METH on pre- and post- synaptic alteration in hippocampus and the protective effect of melatonin. The four-days-old rat pups (P4) were divided into four groups. Rats were subcutaneously injected with 0.9% normal saline (control group), 5-10 µg/kg of METH (METH group), 30 minutes pretreatment of 10 µg/kg melatonin following METH (METH+MEL group), or 10 µg/kg of melatonin (MEL group), respectively, for seven days. Synaptophysin and PSD-95 were used to identify the pre- and post-synaptic alteration in the hippocampus. The results showed that METH significantly reduced both the synaptophysin and PSD-95 level in the hippocampus. It also reduced synaptophysin-immunoreactivity in CA1, CA3, and the dentate gyrus. Interestingly, melatonin could significantly lower the METH effect. In summary, melatonin can attenuate the METH effect by reducing the pre- and post-synapse degeneration in the hippocampus.
Keywords:
Hippocampus,
Melatonin,
Neuroprotection,
in vivo,
synaptic degeneration
Conference:
14th Meeting of the Asian-Pacific Society for Neurochemistry, Kuala Lumpur, Malaysia, 27 Aug - 30 Aug, 2016.
Presentation Type:
Poster Presentation Session
Topic:
14th Meeting of the Asian-Pacific Society for Neurochemistry
Citation:
Leeboongam
T,
Govitrapong
P and
Phansuwan-Pujito
P
(2016). Protective effect of melatonin on methamphetamine-induced synaptic degeneration in postnatal hippocampus.
Conference Abstract:
14th Meeting of the Asian-Pacific Society for Neurochemistry.
doi: 10.3389/conf.fncel.2016.36.00187
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Received:
04 Aug 2016;
Published Online:
11 Aug 2016.
*
Correspondence:
Prof. Pansiri Phansuwan-Pujito, Srinakharinwirot University, Department of Anatomy, Faculty of Medicine, Bangkok, 10110, Thailand, pansiri@swu.ac.th