Event Abstract

Back to Event

Immunoregulatory activity of Decidual/Endometrial Stromal Cells in humans

Carolina Reyes-Perdomo1, 2*, Karol Torres1, Enrique García Olivares3, Pedro Silva4, Marcos Lopez1, Wendy Nieto1 and Osmany Blanco Muñoz1
  • 1 Fundación Cardiovascular de Colombia, Biotecnología, Innovación y Desarrollo Tecnológico, Colombia
  • 2 Pontificia Universidad Javeriana, Colombia
  • 3 Instituto de Biopatología y Medicina Regenerativa, Universidad de Granada, Spain
  • 4 Universidad de Santander, Colombia

Decidua is the maternal tissue that is in close contact with the fetal trophoblast cells. During normal pregnancy tolerance mechanisms develop at the level of the decidua. In the case of abortion or pre-eclampsia caused by antiphospholipid antibodies or autoimmune diseases, an immunologic activation develops against trophoblast. Human decidual cells (DSC) from the uterus and parent cells, the endometrial cells (ESC), are closely related to the mesenchymal stem cells (MSC), and like the latter, can survive during xenotransplants, and probably during semiallogeneic transplants as well. DSC/ESC show advantages over bone marrow MSC in terms of collection. Additionally, they have surface antigen markers in common like HLA-G, due to their immunomodulatory activity. In our present work it has been proved that the DSC/ESC might be the responsible for the immunomodulatory effects over CD4+ CD25+ FOXP3+ nTreg cells when they are in cell-cell contact. This contact is consistent with an increased intensity of CD25 expression on CD4+ T cells.

METHODS: DSC were collected from termed placenta and ESC were isolated from menstruation. Samples were purified and cultured. To study T cells proliferation and diferentation, T cells were isolated from peripheral blood from healthy donors and co-cultured with DSC/ESC. Immunomodulatory effects of the DSC/ESC were determined through monoclonal antibodies by flow cytometry.

RESULTS: It has been proved that the DSC/ESC might be the responsible for the immunomodulatory effects over CD4+ CD25+ FOXP3+ nTreg cells when they are in cell-cell contact. This contact is consistent with an increased intensity of CD25 expression on CD4+ T cells.

CONCLUSION: HLA-G expressed by the DSC might be responsible for the immunomodulatory effects of DSC in Treg cells, proving its importance as immunosuppressive. Although ESC were negative HLA-G, they have an immunomudulatory effect over CD4+ cells. Further studies are necessary to elucidate the mechanism underlying this modulation.

This results may be useful for clinical therapies or gynecological-obstetrician immunological problems.

Acknowledgements

We thank Clínica Materno Infantil San Luis S.A. for their help in recruiting placental donors. We also thank University of Santander (UDES).

This work is supported by grants from Departamento Administrativo de Ciencia, Tecnología e Innovación (COLCIENCIAS).

References

1. Haddad E.L. 2011. Mesenchimal Stem Cells: Immunology and therapeutic benefits, Stem Cells in Clinic and Research, Dr. Ali Gholamrezanezhad (Ed.). InTechOpen. p. 60-82.
2. Spaggiari, G.M & Moretta, L. 2013. Cellular an molecular interactions of mesenchymal stem cells in innate immunity. Immunology and Cell Biology 91. Nature. p. 27-31.
3. Blanco, O.; Leno-Durán, E.; Morales, JC.; Olivares, EG. & Ruiz-Ruiz, C. 2009. Human Decidual Stromal Cells protect lymphocytes from apoptosis. Placenta 30. ElSevier. p. 677-685.
4. Blanco, O.; Tirado, I.; Muñoz-Fernández, R.; Abadía-Molina, AC.; García-Pacheco, JM.; Peña, J & Olivares EG. 2008. Human decidual stromal cells express HLA-G. Human Reprod 23. p. 144-152.
5. Benvenuto, F.; Ferrari S.; Gerdoni, E.; Gualandi, F.; Frassoni, F.; Pistoia, V.; Mancardi, G. & Uccelli, A. 2007. A human mesenchymal stem cells promote survival of T cells in a quiescent state. Stem Cells 25. p. 1753-1760.
6. Uccelli A.; Moretta, L.; Pistoia, V. 2008. Mesenchymal stem cells in health and disease. Nat Rev Immunol 8. p. 726-736.
7. García-Pacheco JM.; Oliver, C.; Kimatrai, C.; Blanco, FJ. & Olivares EG. 2001. Human decidual stromal cells express CD34 and STRO-1 and are related eith bone marrow stromal precursors. Mol Hum Reprod 7. p. 151-157.
8. Hida N.; Nishiyama, N.; Miyoshi, S.; Kira, S.; Segawa, K.; Uyama, T.; Mori, T.; Miyado, K.; Ikegami, y.; Cui, C.; Kiyono, T.; Kyo, S.; Shimizu, T.; Okano, T.; Sakamoto, M.; Ogawa S. & Umezawa, A. 2008. Novel cardiac precursor-like cells from human menstrual blood-derived mesenchymal cells. Stem Cells 26. p. 1695-1704.

Keywords: Decidual Stromal cells, Endometrial Stromal Cells, HLA-G, Treg cells, immumodulation

Conference: 15th International Congress of Immunology (ICI), Milan, Italy, 22 Aug - 27 Aug, 2013.

Presentation Type: Abstract

Topic: Translational immunology and immune intervention

Citation: Reyes-Perdomo C, Torres K, García Olivares E, Silva P, Lopez M, Nieto W and Blanco Muñoz O (2013). Immunoregulatory activity of Decidual/Endometrial Stromal Cells in humans. Front. Immunol. Conference Abstract: 15th International Congress of Immunology (ICI). doi: 10.3389/conf.fimmu.2013.02.01185

Copyright: The abstracts in this collection have not been subject to any Frontiers peer review or checks, and are not endorsed by Frontiers. They are made available through the Frontiers publishing platform as a service to conference organizers and presenters.

The copyright in the individual abstracts is owned by the author of each abstract or his/her employer unless otherwise stated.

Each abstract, as well as the collection of abstracts, are published under a Creative Commons CC-BY 4.0 (attribution) licence (https://creativecommons.org/licenses/by/4.0/) and may thus be reproduced, translated, adapted and be the subject of derivative works provided the authors and Frontiers are attributed.

For Frontiers’ terms and conditions please see https://www.frontiersin.org/legal/terms-and-conditions.

Received: 18 Jul 2013; Published Online: 22 Aug 2013.

* Correspondence: Miss. Carolina Reyes-Perdomo, Fundación Cardiovascular de Colombia, Biotecnología, Innovación y Desarrollo Tecnológico, Floridablanca, Santander, 00000, Colombia, caroreyes81@yahoo.com