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Association study among HLA, KIR, MICA, PARK2, and cytokine genes in patients with leprosy from Southern Brazil

Priscila S. Mazini1*, Danilo A. Francechi1, Pãmela G. Reis1, Ana M. Sell1 and Jeane E. Visentainer1
  • 1 State University Maringá, Biosciences Applied to Pharmacy, Brazil

Polymorphisms of HLA, KIR, MICA, PARK and cytokine genes were analysed in patients with leprosy and healthy volunteers from Brazil. HLA, KIR and MICA genes were genotyped by PCR-SSO. PARK2-2599 polymorphism was investigated by real time PCR. PCR-SSP was performed for: TNF-308/-238; IL2-330/+166; IL6-174; IFNG+874; TGFB1+869/+915; and IL10-592/-819/-1082. HLA-DRB1*16 was associated to per se leprosy (P=0.0059), tuberculoid and dimorph forms (P=0.016 and P=0.001, respectivelly), while the HLA-DRB1*16:02 was associated to lepromatous form (P=0.023). KIR2DS2 and KIR2DS3 frequencies were higher in tuberculoid leprosy when compared to lepromatous leprosy. Analysis of KIR-ligands reveled KIR2DL1-C2/C2 elevated in tuberculoid in comparison to all other leprosy subgroups and controls. A negative association between KIR2DL3-C1 and KIR2DL3-C1/C1 and the tuberculoid group was identified. Borderline patients exhibited a higher frequency of KIR3DL2-A3/11 than the controls and lepromatous patients, and a lower frequency of KIR2DL1-C2 than the controls and tuberculoid subgroup. MICA*027 allele was decreased (P= 0.01) in leprosy patients and MICA*010 (P = 0.05) and MICA*027 alleles (P = 0.01) in multibacillary leprosy patients compared to the control group. No significant difference of genotype frequencies among the groups for PARK2 gene was observed. For TNF-308, a higher frequency of GG genotype (P = 0.009), along with a decreased frequency of GA/AA genotypes was observed among leprosy patients as compared to the control group (P = 0.009). Analysis of IL10 genotypes revealed lower frequency of GCC/GCC haplotype in lepromatous leprosy patients (6.2%) in comparison to controls (15.4%). The study suggests the involvement of immune response genes in leprosy.

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Keywords: Genes, Leprosy, immunology, innate immune response, association studies

Conference: 15th International Congress of Immunology (ICI), Milan, Italy, 22 Aug - 27 Aug, 2013.

Presentation Type: Abstract

Topic: Innate immunity

Citation: Mazini PS, Francechi DA, Reis PG, Sell AM and Visentainer JE (2013). Association study among HLA, KIR, MICA, PARK2, and cytokine genes in patients with leprosy from Southern Brazil. Front. Immunol. Conference Abstract: 15th International Congress of Immunology (ICI). doi: 10.3389/conf.fimmu.2013.02.01157

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Received: 19 Jul 2013; Published Online: 22 Aug 2013.

* Correspondence: Dr. Priscila S Mazini, State University Maringá, Biosciences Applied to Pharmacy, Maringá, Brazil, priscilasaamara@gmail.com