Combination therapy of methotrexate with ellagic acid attenuates the over expression of Pro-inflammatory Cytokines and modulates antioxidant status in collagen induced arthritis
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1
Department of toxicology, Jamia Hamdard, India
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2
Jamia Millia Islamia, Department of Biotechnology, India
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3
Department of Pharmacognosy, Jamia Hamdard, India
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4
Ayurvedic Research Lab., Dabur Research centre, Ghaziabad, U.P, India
The inclusion of antioxidant for the treatment of arthritis, especially under the therapy with immune-suppressant, is motivated because antioxidant plays an essential role in disease progression and moreover, immunosuppressive treatment suffers redox homeostasis balance of the organism. The present study was designed to evaluate the combinatory effect of methotrexate (MTX) and ellagic acid (EA) on the progression of collagen induced arthritis. Arthritis was induced in Wistar rats by collagen induced arthritis (CIA) method. CIA rats were treated with methotrexate (1 mg / kg / week) and EGCG (60 mg / kg) daily, and combination of MTX and EGCG for a period of 28 days. Paw swelling changes, scoring and histopathological was assessed to evaluate the antiarthritic effect. The effects of treatment in the rats were assessed by biochemical (articular elastase, MPO, LPO, GSH, Catalase, SOD and NO) in joint tissue homogenate to observe the modulation of antioxidant and inflammatory status, inflammatory mediators (IL-1β, TNF-α, IL-10, and PGE2) and histological studies in joints. MTX and EA combination potentiated the antiarthritic (decrease of hind paw volume and scoring) and the antioxidant effect (GSH, Catalase, SOD) as well as suppression of lipid peroxidation. Combination therapy of MTX and EA significantly inhibited the development phase of arthritis, which is supported by histopathological and attenuation of pro inflammatory cytokines. EA act as potent antioxidant and immunomodulator, suggesting that combined administration of MTX along with EA suppressed the development of arthritic progression in rats.
Acknowledgements
Author is grateful to ICMR, GOI for providing Research Associateship (RA).
Keywords:
Ellagic Acid,
Rheumatoid arthritis,
Inflammation,
Cytokines,
articular elastase
Conference:
15th International Congress of Immunology (ICI), Milan, Italy, 22 Aug - 27 Aug, 2013.
Presentation Type:
Abstract
Topic:
Translational immunology and immune intervention
Citation:
Umar
S,
Ahmad
S,
Katiyar
CK and
Khan
HA
(2013). Combination therapy of methotrexate with ellagic acid attenuates the over expression of Pro-inflammatory Cytokines and modulates antioxidant status in collagen induced arthritis.
Front. Immunol.
Conference Abstract:
15th International Congress of Immunology (ICI).
doi: 10.3389/conf.fimmu.2013.02.00795
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Received:
14 Jun 2013;
Published Online:
22 Aug 2013.
*
Correspondence:
Dr. Sadiq Umar, Department of toxicology, Jamia Hamdard, New Delhi, Delhi, 110062, India, sadiq36@gmail.com