Granzyme A is an additional marker for tuberculosis (TB) to discriminate between patients with active disease and subjects with latent infection.
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1
Dipartimento di Biopatologia e Biotecnologie Mediche e Forensi, University of Palermo, Italy
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2
L. Spallanzani” National Institute for Infectious Diseases (INMI), IRCCS, Translational Research Unit, Department of Epidemiology and Preclinical Research, Italy
Tuberculosis is a major global health problem, with an estimated 8.7 million cases and 1.4 million deaths in 2011. Cytotoxic molecules such as granulysin, perforin and granzymes produced by cytolytic T cells directly contributes to immune defense against tuberculosis (TB). It is still difficult to discriminate active disease, latent infection (LTBI), past TB patients subjects based on Quantiferon TB-Gold in tube (QFT-IT) response; therefore the aim of this study was to evaluate if the responses to granzyme A-could help to discriminate between the different TB stages. We compared the release of granzyme A in whole blood cells stimulated with QFT-IT. We found significant higher level of this cytototoxic molecule in LTBI and past TB subjects compared to the level found in patients with active disease (at least p<0.001).
These results suggest that the evaluation of the level of cytotoxic molecules such as granzyme A could be considered as a biomarker of TB to better discriminate among LTBI, active disease, past TB.
Acknowledgements
This work was supported by grants from the European Commission within the 6th Framework Programme, TB-VAC contract no. LSHP-CT-2003-503367 and the 7th Framework Programme, NEWTBVAC contract no. HEALTH-F3-2009-241745 (The text represents the authors' views and does not necessarily represent a position of the Commission who will not be liable for the use made of such information).
Keywords:
Tuberculosis,
granzyme A,
ELISA,
LTBI,
Cytotoxic molecules,
TB patients
Conference:
15th International Congress of Immunology (ICI), Milan, Italy, 22 Aug - 27 Aug, 2013.
Presentation Type:
Abstract
Topic:
Host-pathogen interactions
Citation:
Caccamo
N,
Orlando
V,
La Manna
M,
Di Liberto
D,
Cutrera
S,
Dieli
F,
Vanini
V,
Petruccioli
E and
Goletti
D
(2013). Granzyme A is an additional marker for tuberculosis (TB) to discriminate between patients with active disease and subjects with latent infection..
Front. Immunol.
Conference Abstract:
15th International Congress of Immunology (ICI).
doi: 10.3389/conf.fimmu.2013.02.00683
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Received:
13 Jun 2013;
Published Online:
22 Aug 2013.
*
Correspondence:
Prof. Nadia Caccamo, Dipartimento di Biopatologia e Biotecnologie Mediche e Forensi, University of Palermo, Palermo, Italy, nadia.caccamo@unipa.it