Large zinc finger transcription factor Schnurri-3 functions in the osteochondroprogenitor cell to regulate bone mass
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1
NCATS-NIH, United States
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2
Merck Pharmaceuticals, United States
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3
Weill Cornell Medical College, United States
The large zinc finger transcription factor Schnurri-3 (Shn3) was originally identified as a DNA binding protein of the heptameric recombination signal sequence for V(D)J recombination of immunoglobulin genes. Previous studies from our group have revealed an unexpected role for Shn3 in the skeletal system and have identified Shn3 as a central regulator of postnatal bone mass. Mice lacking Shn3 display severe osteosclerosis primarily due to enhanced osteoblast activity. In this study we have characterized a specific cell population in which Shn3 functions to regulate bone mass. Based on analysis of mice lacking Shn3 in osterix or collagen II expressing cells, we hypothesized that Shn3 must be functioning in the common progenitor cell for osteoblasts and chondrocytes-the osteochondroprogenitor cell. However, characterization of this cell population has been limited due to lack of definitive cell surface markers to phenotype these cells. We established a flow cytometry based high throughput screen to identify a panel of unique cell surface molecules that can be used to identify and isolate osteochondroprogenitor cells. These osteochondroprogenitor cells not only formed bone and cartilage in vitro but also in vivo when transplanted under the kidney capsule of recipient donors. Lastly, we were able to demonstrate that Shn3 functions intrinsically in the osteochondroprogenitor cells to control bone mass.
Keywords:
osteoprogenitor cell,
Schnurri-3,
Bone,
osteoblast,
bone stem cell
Conference:
15th International Congress of Immunology (ICI), Milan, Italy, 22 Aug - 27 Aug, 2013.
Presentation Type:
Abstract
Topic:
Translational immunology and immune intervention
Citation:
SINGH
A,
Jones
D and
Glimcher
L
(2013). Large zinc finger transcription factor Schnurri-3 functions in the osteochondroprogenitor cell to regulate bone mass.
Front. Immunol.
Conference Abstract:
15th International Congress of Immunology (ICI).
doi: 10.3389/conf.fimmu.2013.02.00563
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Received:
22 Apr 2013;
Published Online:
22 Aug 2013.
*
Correspondence:
Dr. ANJU SINGH, NCATS-NIH, Rockville, United States, ANJU.SINGH@NIH.GOV
Dr. Dallas Jones, Merck Pharmaceuticals, Boston, United States, dallas.jones@merck.com