Expression of selected Th1/Th2 transcription factors and immune-response genes in cerebral ischemia and senescence
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1
Institute of Molecular Biology, National Academy of Science of Republic of Armenia, Armenia
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2
Faculty of Medicine and Dentistry Palacky University, Institute of Molecular and Transplantation Medicine, Czechia
T helper (Th) subsets have been implicated in ischemic stroke. This study, therefore, aimed to assess functional state of Th1/Th2 cells by measuring expression of genes encoding GATA3 and TBX21, markers of Th2 and Th1, respectively, in stroke-affected and healthy control subjects. 54 patients with ischemic stroke (mean age±SE: 71.91±1.47 years) on 1st day after stroke onset, 28 aged (66.71±1.5) and 46 middle-aged (37.09±1.04) healthy controls of Armenian ancestry were enrolled with ethical clearance. mRNA expression in peripheral blood cells was determined by quantitive real-time polymerase chain reaction (RT-PCR) with PSMB2 as housekeeping gene. There was no difference in GATA3 mRNA expression between the investigated groups (p>0.05). However, decreased TBX21 mRNA expression was observed in ischemic stroke patients and in aged controls (3.1 and 2.9 fold, respectively; p<0.05) indicating that these changes may be age-dependent. Aging process is a major risk factor for cerebral ischemia and numbers&function of Th1 cells decrease while those of Th2 cells increase during aging. Thus, in further experiments we analysed peripheral blood expression levels of IL2 and IL2RG. Both IL2 and IL2RG mRNA transcripts were downregulated in patients and aged controls compared to middle-aged controls. Moreover, there was a positive correlation between the expression of TBX21, IL2 and IL2RG. In summary, GATA3 expression levels were not altered by ischemic stroke condition or aging processes, whereas TBX21, IL2 and IL2RG were upregulated in ischemic stroke.
Acknowledgements
This study was supported by the IGA PU LF 2013_009 and CZ.1.05./2.1.00/01.0030, and the National Academy of Sciences of Armenia.
Keywords:
Brain Ischemia,
Aging,
Th1,
th2,
TBX21,
GATA3,
ischemic stroke
Conference:
15th International Congress of Immunology (ICI), Milan, Italy, 22 Aug - 27 Aug, 2013.
Presentation Type:
Abstract
Topic:
Immune-mediated disease pathogenesis
Citation:
Hakobjanyan
A,
Boyajyan
A,
Hovsepyan
L,
Navratilova
Z,
Klevcova
P and
Petrek
M
(2013). Expression of selected Th1/Th2 transcription factors and immune-response genes in cerebral ischemia and senescence.
Front. Immunol.
Conference Abstract:
15th International Congress of Immunology (ICI).
doi: 10.3389/conf.fimmu.2013.02.00249
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Received:
14 Mar 2013;
Published Online:
22 Aug 2013.
*
Correspondence:
Prof. Anna Boyajyan, Institute of Molecular Biology, National Academy of Science of Republic of Armenia, Yerevan, Armenia, aboyajyan@sci.am
Prof. Martin Petrek, Faculty of Medicine and Dentistry Palacky University, Institute of Molecular and Transplantation Medicine, Olomouc, Czechia, martinpe@email.cz