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T Lymphocyte-mediated suppression of murine granulopoiesis by oral allergen pretreatment of sensitized/challenged mice

Maria Ignez Gaspar-Elsas1*, Tulio Queto1, Ricardo Luz2, Bianca DeLuca1, Cassio Almeida2, Daniela Masid De Brito2, Renato Ferreira1, Priscila Cauduro2, Bruno Vieira1, 2 and Pedro Xavier-Elsas2
  • 1 FIOCRUZ, Brazil
  • 2 Universidade Federal do Rio de Janeiro, immunology, Brazil

Oral ovalbumin pre-exposure (oral OVA) effectively prevents the subsequent allergic pulmonary inflammation in sensitized/challenged (S/C) mice, reducing lung eosinophil (Eos) infiltration. In OVA-S/C mice, eosinopoiesis is suppressed by a proapoptotic mechanism requiring iNOS and CD95L. The role of bone-marrow (BM) regulation by iNOS/CD95L in the effects of oral OVA, remains to be established. We examined oral OVA effects on granulopoiesis in S/C mice. BP-2, BALB/c and C57BL/6 wild-type (WT) mice, and mutants lacking CD95L, were given OVA in water, and S/C with OVA. Granulopoiesis was evaluated in liquid and semisolid BM cultures. Spleen T cells from oral OVA/S/C donors, or from S/C controls, were transferred to syngeneic recipients, which were subsequently S/C with OVA before evaluation of BM eosinopoiesis. Oral OVA/S/C BP-2 mice had significantly decreased, relative to oral Water controls: a) Eos numbers in BM; b) IL-5-stimulated eosinopoiesis and GM-CSF-stimulated neutropoiesis; c) GM-CSF-stimulated Eos colony formation; d) OVA-specific IgE. Oral OVA was effective in BM CD95L-deficient mice. The effects of oral OVA on BM Eos numbers were duplicated by transfer of splenic T cells from oral OVA/S/C donors to syngeneic recipients, which were subsequently S/C, demonstrating a novel regulatory effect of oral OVA-induced T cells on granulopoiesis.

Acknowledgements

CNPq-FAPERJ-CAPES.

References

Elsas, PX, Maximiano, ES, Vargaftig, BB, Elsas, MI. (2003). The effects of allergen and anti-allergic drugs on murine hemopoietic cells: moving targets, unusual mechanisms, and changing paradigms. Current Drug Targets - Inflammation & Allergy 2, 329-337.
Gaspar Elsas, MI, Maximiano, ES, Joseph, D, Alves, L, Topilko, A, Vargaftig, BB, Xavier Elsas, P. (2000a). Upregulation by glucocorticoids of responses to eosinopoietic cytokines in bone-marrow from normal and allergic mice. Br J Pharmacol, 129, 1543-1552.
Gaspar Elsas, MI, Joseph, D, Lintomen, L, Maximiano, ES, Bodstein, M, Xavier Elsas, P, Vargaftig, BB. (2000b). Murine myeloid progenitor responses to GM-CSF and eosinophil precursor responses to IL-5 represent distinct targets for downmodulation by prostaglandin E(2). Br J Pharmacol 130, 1362-1368.
Jones, CP, Paula Neto, HA, Assreuy, J, Vargaftig, BB, Gaspar Elsas, MI, Elsas, PX. (2004). Prostaglandin E2 and dexamethasone regulate eosinophil differentiation and survival through a nitric oxide- and CD95-dependent pathway. Nitric Oxide 11, 184-93.
Lintomen, L, Gaspar-Elsas, MI, Maximiano, ES, de Paula Neto, HA, Joseph, D, Vargaftig, BB, Elsas, PX. (2002). Allergenic sensitization prevents upregulation of haemopoiesis by cyclo-oxygenase inhibitors in mice. Br J Pharmacol 135, 1315 -1323.
Ten, RM, Pease, RL, McKean, DJ, Bell, MP, Gleich, GJ. (1989). Molecular cloning of the human eosinophil peroxidase. Evidence for the existence of a peroxidase multigene family. J Exp Med 169, 1757–1769.
Rodrigues, C. M., O.A. Martins-Filho, N.M. Vaz, C.R. Carvalho (2006). Systemic effects of oral tolerance on inflammation: mobilization of lymphocytes and bone marrow eosinopoiesis Immunology 117, 517–525.
Gaspar Elsas MI, Joseph D, Elsas PX, Vargaftig BB. Rapid increase in bone-marrow eosinophil production and responses to eosinopoietic interleukins triggered by intranasal allergen challenge. Am J Respir Cell Mol Biol 1997; 17 (4):404–13.

Keywords: allergy, tolerance, eosinophil, Hematopoiesis, mucosal immunity

Conference: 15th International Congress of Immunology (ICI), Milan, Italy, 22 Aug - 27 Aug, 2013.

Presentation Type: Abstract

Topic: Adaptive Immunity

Citation: Gaspar-Elsas M, Queto T, Luz R, DeLuca B, Almeida C, Masid De Brito D, Ferreira R, Cauduro P, Vieira B and Xavier-Elsas P (2013). T Lymphocyte-mediated suppression of murine granulopoiesis by oral allergen pretreatment of sensitized/challenged mice. Front. Immunol. Conference Abstract: 15th International Congress of Immunology (ICI). doi: 10.3389/conf.fimmu.2013.02.00129

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Received: 10 Mar 2013; Published Online: 22 Aug 2013.

* Correspondence: Dr. Maria Ignez Gaspar-Elsas, FIOCRUZ, Rio de Janeiro, Brazil, gasparelsas@gmail.com