Chemical modification of Cladophora nanocellulose to provide a non-toxic material with anticoagulant properties
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1
Uppsala University, Nanotechnology and Functional Materials, Department of Engineering Sciences, Sweden
Blood purification is a common procedure for treating patients with kidney failure in which an extracorporeal device filled with a porous material containing antibodies can retain toxins from the blood. In order to avoid activation and coagulation, heparin is administrated to patients; however this sulfonated polysaccharide may cause many side effects such as intense bleeding and osteoporosis. In this project nanocellulose from Cladophora green algae was used for the development of a porous material with anticoagulant properties. A periodate oxidation followed by an amine cross-linking and subsequent reduction produced dialdehyde cellulose beads ranging from 10-100 μm in diameter with improved mechanical properties and high stability in alkaline media. This material was then sulfonated to acquire anticoagulant properties and characterized by FTIR, ζ-potential, condutometric titration, elemental analysis and BET surface area showing that its porosity varies with the degree of sulfonation. After extensive washing, toxicology experiments were performed with a THP-1 monocyte cell line in order to examine if the material was non-toxic and could be suitable as a matrix in blood purification.
Science Without Boarders Scholarship, CAPES, Brazilian Ministry of Education
References:
[1] Lindh J, Carlsson DO, Strømme M, Mihranyan A. Convenient one-pot formation of 2,3-dialdehyde cellulose beads via periodate oxidation of cellulose in water. Biomacromolecules. 2014 May 12;15(5):1928-32
Keywords:
biomaterial,
nanofiber,
Polymeric material,
biomacromolecule
Conference:
10th World Biomaterials Congress, Montréal, Canada, 17 May - 22 May, 2016.
Presentation Type:
Poster
Topic:
Nano-structured materials for unique functions
Citation:
Rocha
I,
Ferraz
N,
Mihranyan
A,
Strömme
M and
Lindh
J
(2016). Chemical modification of Cladophora nanocellulose to provide a non-toxic material with anticoagulant properties.
Front. Bioeng. Biotechnol.
Conference Abstract:
10th World Biomaterials Congress.
doi: 10.3389/conf.FBIOE.2016.01.01690
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Received:
27 Mar 2016;
Published Online:
30 Mar 2016.
*
Correspondence:
Dr. Igor Rocha, Uppsala University, Nanotechnology and Functional Materials, Department of Engineering Sciences, Uppsala, Sweden, igor.rocha@angstrom.uu.se
Dr. Natalia Ferraz, Uppsala University, Nanotechnology and Functional Materials, Department of Engineering Sciences, Uppsala, Sweden, Natalia.Ferraz@angstrom.uu.se
Dr. Albert Mihranyan, Uppsala University, Nanotechnology and Functional Materials, Department of Engineering Sciences, Uppsala, Sweden, albert.mihranyan@angstrom.uu.se
Dr. Maria Strömme, Uppsala University, Nanotechnology and Functional Materials, Department of Engineering Sciences, Uppsala, Sweden, Maria.Stromme@angstrom.uu.se
Dr. Jonas Lindh, Uppsala University, Nanotechnology and Functional Materials, Department of Engineering Sciences, Uppsala, Sweden, Jonas.Lindh@angstrom.uu.se