The inflammatory response to implanted materials, fibrous capsule characterisation
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1
UNSW Australia, Graduate School of Biomedical Engineering, Australia
A major problem associated with the implantation of foreign materials is their propensity to induce inflammation and fibrosis. While the infiltration of inflammatory cells and formation of a fibrous capsule around the implanted material are relatively well characterised, research into the mechanistic events that occur are still relatively unknown. Recruitment of mast cells, to sites of material implantation is thought to be involved in the recruitment of inflammatory cells, through the release of pro-inflammatory proteases and cytokines on activation from their α-granules. This study aims to investigate the role that mast cells have on the inflammatory response to implanted materials and fibrous capsule formation.
Test materials for implantation consisted of the naturally occurring polymer Chitosan, with and without acid pre-treatment, and Surgicel, a commercially available cellulose haemostatic agent. Materials were subcutaneously implanted into rats for 7 days and following explanation histological characterisation was carried out through H&E, picrosirrus red and Leder stain, as well as immunohistochemistry.
Results showed the inflammatory response varied between the different material implants, as indicated by the thickness of the fibrous capsule and distribution of collagen. Presence and location of Leder stain positive cells were shown within the fibrous capsule in response to all material implants. The mast cell receptor, c-kit, as well as proteoglycans (PGs) and glycosaminoglycan (GAGs) known to be produced by mast cells, serglycin, perlecan and chondroitin sulphate, were shown to be expressed with the fibrous capsule, with differing expression and distribution, as well as co-localisation with other markers. These data support the role that mast cells play in the inflammatory host response to material implants, where mediators released from their α-granules impact on the formation of a collagen dense fibrous capsule. Highlighting the importance of ECM characterisation in addition to collagen, and the potential use of PGs as inflammatory markers.
Dr Simon McCarthy; Dr Barbara McGrath
Keywords:
biomaterial,
Implant
Conference:
10th World Biomaterials Congress, Montréal, Canada, 17 May - 22 May, 2016.
Presentation Type:
Poster
Topic:
Biomaterials in immune response
Citation:
Farrugia
B,
Whitelcok
J and
Lord
M
(2016). The inflammatory response to implanted materials, fibrous capsule characterisation.
Front. Bioeng. Biotechnol.
Conference Abstract:
10th World Biomaterials Congress.
doi: 10.3389/conf.FBIOE.2016.01.00994
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Received:
27 Mar 2016;
Published Online:
30 Mar 2016.