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Investigation of the BMP-2 adsorption to different solvent-treated 3D PCL scaffolds

Alicja Kosik1, Cedryck Vaquette2, Elizabeth Graham3, Adrian Chlanda1, Sudhees Kumar4, Saso Ivanovski4, Wojciech Swieszkowski1 and Dietmar Hutmacher2
  • 1 Warsaw University of Technology, Faculty of Materials Science and Engineering, Poland
  • 2 Queensland University of Technology, Institute of Health and Biomedical Innovation, Australia
  • 3 Queensland University of Technology, Institute of Future Environment, Australia
  • 4 Griffith University, Griffith Health Institute, Australia

Introduction: The application of bone morphogenic proteins (BMPs) is a prominent area of research for application in bone tissue engineering. This biological protein is generally combined with a gel or a collagenous carrier to facilitate its delivery. However, this results in poorly controlling the release of the bioactive cue potentially leading to significant adverse effects. A novel strategy involves the absorption of the BMP onto a biomaterial scaffold in order to alter the release kinetic. In this study, we investigated the impact of surface modification of polycaprolactone (PCL) 3D printed scaffolds onto BMP-2 adsorption efficacy and release.

Materials and Methods: The scaffold were 3D printed using an in house Fused Deposition modelling equipment. The scaffold surface modification was achieved by immersing the construct into a solvent (acetone) of the PCL or by depositing a layer of calcium phosphate in order to increase the roughness and the surface area per volume available for the BMP adsorption. Following surface modification, BMP-2 was adsorbed to 3D printed PCL scaffolds (non modified, solvent treatments and Calcium Phosphate treatment) by immersion in a  0.1 μg/μL BMP-2 solution for 1h at room temperature.

Results and Discussion: Acetone treatments increased significantly material roughness and surface area as measured by AFM and BET. It was also demonstrated that the surface modification significantly impact on the wettability of the scaffold. After exposure scaffolds in BMP-2 solution, the highest adsorption efficiency was found for the acetone treated group which had around 20-30% of BMP adsorbed, which is consistent with previous studies.

Conclusion: We demonstrated that adsorption of BMP-2 can be achieved in treated 3D printed scaffolds and  resulting in a maximum of  30% of BMP attached to the scaffold.

References:
[1] Darilis Sua´rez-Gonza´lez et. all, "Controlled Multiple Growth Factor Delivery from Bone Tissue Engineering Scaffolds via Designed Affinity", Tissue engineering: Part A Volume 20,15-16, 2014
[2] Janki J. Patel, "Bone Morphogenetic Protein-2 Adsorption onto Poly-e-caprolactone Better Preserves Bioactivity In Vitro and Produces More Bone In Vivo than Conjugation Under Clinically Relevant Loading Scenarios", Tissue engineering: Part C Volume 21,5, 2015

Keywords: biomaterial, growth factor, protein, 3D scaffold

Conference: 10th World Biomaterials Congress, Montréal, Canada, 17 May - 22 May, 2016.

Presentation Type: Poster

Topic: Biomimetic materials

Citation: Kosik A, Vaquette C, Graham E, Chlanda A, Kumar S, Ivanovski S, Swieszkowski W and Hutmacher D (2016). Investigation of the BMP-2 adsorption to different solvent-treated 3D PCL scaffolds. Front. Bioeng. Biotechnol. Conference Abstract: 10th World Biomaterials Congress. doi: 10.3389/conf.FBIOE.2016.01.00611

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Received: 27 Mar 2016; Published Online: 30 Mar 2016.